The scepter cell counter delivers rapid cell counts in a handheld easy to use format. When you have 20 animal bal counts to do, it saves you a lot of time plus your sanity. Onscreen instructions will walk user through assay, step by step in less than 20 seconds on average the scepter cytometer will display all the data needed regarding the culture after the count is complete, use the toggle button to move from one histogram to another, manipulate, or gate counts. Easily capture, analyze and manipulate data sets with scepter software pro.
Now you can liberate yourself from the microscope and avoid the tedium of manual counting. Millipore works together with enduser scientists, architects, consultants, lab managers, facilities managers, contractors. The cell counter is very userfriendly and doesnt take long to master. Millipore is the partner of choice for total water purification systems. Tv and television manuals and free pdf instructions. The e22 led monitor is equipped with a threepronged grounded plug, a plug with a third grounding pin. Merck millipore is the partner of choice for total water purification systems. In less than 20 seconds on average the scepter cytometer will. Smith, sonia gil, grace johnston bioscience division, millipore inc.
Data can be downloaded onto your computer, so thats a plus as well. Scepter sensor technology compatible with 60 m and 40 m sensors, the scepter 2. Find msds or sds, a coa, data sheets and more information. Use the 40 m sensor for particles between 3 and 17 m. Millipore millipore milliq synthesis water purification system is in excellent condition. This user manual is a guide for use during the installation, normal operation and maintenance of a synergy or synergy uv water purification system. Determining cell number during cell culture using the scepter cell. The scepter cell counter uses impedancebased particle detection to reliably and precisely count every cell in a sample. The 7 types of systems in the rioselix range are all described together in this manual the guide which is located either at the left or right of each page allows you to see which information is. Please fill out this scepter registration form to receive regular updates on your instruments software and firmware. Application note human pbmc isolation and counting using the.
Terminology the term milliq integral water purification system is replaced by the term milliq system for the remainder of this user manual unless otherwise noted. Counting yeast cells for brewing and wine industries can be. Sample layering before and after ficoll density gradient centrifugation. Synergy is used in this manual to refer to either the synergy or the synergy uv unless otherwise noted. In addition to cell concentration, the scepter screen displays a histogram showing the size or volume distribution of the cell population. If this user manual is not the correct one for your water purification system, then please contact millipore. Are you an existing scepter user interested in upgrading your. Count subpopulations in peripheral blood mononuclear cells pbmc preps. Find the user manual you need for your tv and more at manualsonline. Milliq academic and milliq academic a10 user manual.
Jun 29, 2010 over the past few years biorad, invitrogen, nexcelom, and millipore have introduced the tc10, countess, cellometer, and scepter automated cell counters, respectively. Application note precise and accurate bead counting using the. The scepter instrument miniaturizes coulter cell counting technology found in much larger. Its on screen instructions guide you through the sampling process and in less.
Shop online for a wide selection of merck millipore scepter 2. With its microfabricated, precisionengineered sensor, the scepter cell counter does all the work and delivers accurate and reliable cell counts in less than 30 seconds. The scepter cell counter is a handheld automated device that can be used to count cells. Free sceptre technologies flat panel television user manuals. Millipore works together with end user scientists, architects, consultants, lab managers, facilities managers, contractors. Rapid assessment of size changes using the scepter cell. Operation of the scepter cell counter is similar to using a standard laboratory pipette. The scepter has the smallest profile, since it is handheld, and it is probably the most convenient to use. The scepter cell counter is turned on by depressing and holding the toggle on the back of the instrument. Our emprove program helps you meet the latest regulatory requirements. Automated cell counter by milliporesigma, a business of merck kgaa. Reservoir is used in this manual to refer to the 30 liter pe reservoir, the 60 liter pe reservoir, or the 100 liter pe reservoir unless otherwise noted. The scepter cell counter is the only one on the market to accurately count. Scepter is the only counter on the market to accurately count particles.
Once on, the instrument will prompt the user to attach a sensor. In less than 20 seconds on average the scepter cytometer will display all the data needed regarding the culture. Milliq academicmilliq academic a10 notice the information in this document is subject to change without notice and should not be construed as a commitment by millipore corporation. Since both lower and upper size limits can be set by the user after the cell. The scepter cell counter provides histogram data on cell populations. Scepter, the container industry leader with over 70 years of experience produces everything from gas cans to portable marine gasoline containers and military container products. Counts of 19 cell lines were performed using a z2 coulter counter, the scepter cell counter, an automated visionbased counter such as vicell or countless system, and a hemocytemeter. Millipore scepter handheld automated cell counter medgadget. Over its operating range, the scepter cytometer counts cells more accurately than other automated and manual counting methods when compared to theoretical.
Scepter automated cell counter from millipore biocompare. Millipore corporation announced it has begun shipment of its scepter handheld automated cell counter. Application note precise and accurate bead counting using. Tylanmilliporemykrolis coastal instruments mass flow. Reproducible and reliable counts even with smaller cell types. Data panel houses all data sets used during an analysis session. Application note human pbmc isolation and counting using. Precise volumes of singlecell suspensions are drawn into the sensor at the end of the scepter device. The information contained in this manual is set out in the following manner. While the biorad, invitrogen, and nexcelom instruments rely on the identification and counting of cells by scanning, the scepter uses the same technology as that used. Step into one of our m lab collaboration centers where you can explore, learn, and collaborate. Automated cell counter by milliporesigma, a business of merck kgaa darmstadt germany manufacturer milliporesigma, a business of merck kgaa darmstadt germany 4. Millipore milliq user manual pdf download manualslib.
And if that happens we can look forward to not only having more efficient. Millipores scepter cell counter is designed to count cells in an easytouse, handheld format. Nov 26, 2010 the log of scepter cell counts is plotted against the log of coulter counts, and shows that, across all cell lines tested, scepter counts match coulter counts. Emd millipore is a division of merck kgaa, darmstadt, germany.
The manual loader holds two separate tubes, allowing the user to progress quickly from one sample to another, or to wash between samples following an acquisition. This plug will fit only into a grounded power outlet as a safety feature. Merck millipore works together with end user scientists, architects, consultants, lab managers, facilities managers, contractors and equipment. The quantom tx lgbd10501 microbial cell counter is an imagebased, automated cell counter that can identify and count individual bacterial cells in mere minutes. Merck millipore works together with enduser scientists, architects, consultants, lab managers, facilities managers, contractors and equipment. The scepter cell counter is the only one on the market to accurately count particles smaller than 6. Scepter handheld, automated cell counter millipore cat. Millipore corporation assumes no responsibility for any errors that might appear in this document. The scepter cell counter combines the ease of automated instrumentation and the accuracy of impedancebased particle detection using the coulter emd millipore is a division of merck kgaa, darmstadt, germany figure 1. Use the 60 m sensor for particles between 6 and 36 m. Compatible with 60 m and 40 m sensors, the scepter 2.
Access and display previously stored data as well as files directly uploaded from a connected scepter device. For manual sample handling in a costeffective format, we offer the easycyte instrument with its rotating sample tube loader. Scepter handheld automated cell counter user guide 1 introduction emd millipore corporations patent pending scepter handheld automated cell counter provides a fast and convenient method for counting cells or particles with a disposable sensor. Dec 16, 2009 millipore plans to launch scepter during the latter half of the first quarter of 2010 at a very affordable price. The system employs the coulter principle in a miniaturized, handheld, format enabling rapid cell counting. This user manual is a guide for use during the installation, normal operation, and maintenance of a 30, 60 or 100 liter pe reservoir.